Levels of the interleukins 17A, 22, and 23 and the S100 protein family in the gingival crevicular fluid of psoriatic patients with or without periodontitis,

Abstract Background: Psoriasis and periodontitis are immunologically mediated chronic inflammatory diseases. Epidemiologic evidence has linked both; however, the change of markers in gingival crevicular fluid has been poorly evaluated. Objective: To evaluate the levels of IL-17A, IL-22, IL-23, S100A7, S100A8, and S100A9 in gingival crevicular fluid of psoriatic and healthy subjects with and without periodontitis and their relations to psoriasis severity. Methods: Cross-sectional study. Sample comprised the following groups: healthy controls without periodontitis or with mild periodontitis (n = 21), healthy controls with moderate or severe periodontitis (n = 18), individuals with psoriasis without or mild periodontitis (n = 11), and individuals with psoriasis and moderate or severe periodontitis (n = 32). Levels of IL-17A, IL-22, IL-23, S100A8, and S100A9 were determined by multiplex assay and S100A7 was measured by ELISA. Results: No inter-group differences in the levels of IL-17A, IL-22, IL-23, and S100A7 were found. S100A8 levels were higher in psoriatic patients than controls (p < 0.05). S100A8 was positively correlated with psoriasis severity in the group with psoriasis (p < 0.05). S100A9 exceeded the detection limits. Study limitations: This pilot study presents a small sample size. Conclusions: The concentrations of S100A8 were highest in psoriatic patients regardless of periodontal health/status. S100A8 was associated with the severity of psoriasis. The concentrations of interleukins and S100A7 were similar in psoriatic patients with or without periodontitis vs. healthy controls.

Levels of the interleukins 17A, 22, and 23 and the S100 protein family in the gingival crevicular fluid of psoriatic patients with or without periodontitis.

BACKGROUND: Psoriasis and periodontitis are immunologically mediated chronic inflammatory diseases. Epidemiologic evidence has linked both; however, the change of markers in gingival crevicular fluid has been poorly evaluated. OBJECTIVE: To evaluate the levels of IL-17A, IL-22, IL-23, S100A7, S100A8, and S100A9 in gingival crevicular fluid of psoriatic and healthy subjects with and without periodontitis and their relations to psoriasis severity. METHODS: Cross-sectional study. Sample comprised the following groups: healthy controls without periodontitis or with mild periodontitis (n=21), healthy controls with moderate or severe periodontitis (n=18), individuals with psoriasis without or mild periodontitis (n=11), and individuals with psoriasis and moderate or severe periodontitis (n=32). Levels of IL-17A, IL-22, IL-23, S100A8, and S100A9 were determined by multiplex assay and S100A7 was measured by ELISA. RESULTS: No inter-group differences in the levels of IL-17A, IL-22, IL-23, and S100A7 were found. S100A8 levels were higher in psoriatic patients than controls (p<0.05). S100A8 was positively correlated with psoriasis severity in the group with psoriasis (p<0.05). S100A9 exceeded the detection limits. STUDY LIMITATIONS: This pilot study presents a small sample size. CONCLUSIONS: The concentrations of S100A8 were highest in psoriatic patients regardless of periodontal health/status. S100A8 was associated with the severity of psoriasis. The concentrations of interleukins and S100A7 were similar in psoriatic patients with or without periodontitis vs. healthy controls.

Green Space Exposure Association with Type 2 Diabetes Mellitus, Physical Activity, and Obesity: A Systematic Review.

Type 2 diabetes mellitus (T2DM) is a public health challenge that must be addressed considering the large number of risk factors involved in its appearance. Some environmental risk factors are currently described as predictors of diabetes, with access to green spaces being an element to consider in urban settings. This review aims to study the association between exposure to green spaces and outcomes such as diabetes, obesity, and physical activity in the general population. A systematic review was carried out using the PubMed, Embase, and LILACS databases and other sources. The search strategy was carried out from October 2019 to October 2020. Cross-sectional and cohort studies were included. The article selection was made by a pair of reviewers, and data extraction was carried out using a data extraction sheet. The quality assessment of the included studies was carried out using a validated tool. Finally, 19 scientific articles were included in this review. Evidence supports that people and communities exposed to green spaces, especially in their neighborhood, reduce the risk of T2DM and reduce the risk of being obese and increase the likelihood of physical activity. The onset of T2DM can be moderated by using green spaces, improving physical activity levels, and reducing the risk of being overweight and obese.

Granulomatous pigmented purpuric dermatosis: report of a Latin-American case with blaschkoid distribution.

Granulomatous pigmented purpuric dermatosis clinically manifests as hyperpigmented maculae and petechiae, predominantly on the lower extremities. Histopathologically, it is characterized by a lymphocytic infiltrate in the upper dermis, extravasated erythrocytes, and hemosiderin deposits. There is an infrequent variant called granulomatous pigmented purpuric dermatosis, which histologically is characterized by the presence of non-necrotizing granulomas associated with the classic findings of other pigmented purpuric dermatoses. It more frequently affects middle-aged women of Asian origin, and predominantly on the lower extremities. The authors present the case of a female patient with granulomatous pigmented purpuric dermatosis on the lower extremities with blaschkoid distribution.

Granulomatous pigmented purpuric dermatosis: report of a Latin-American case with blaschkoid distribution

Abstract Granulomatous pigmented purpuric dermatosis clinically manifests as hyperpigmented maculae and petechiae, predominantly on the lower extremities. Histopathologically, it is characterized by a lymphocytic infiltrate in the upper dermis, extravasated erythrocytes, and hemosiderin deposits. There is an infrequent variant called granulomatous pigmented purpuric dermatosis, which histologically is characterized by the presence of non-necrotizing granulomas associated with the classic findings of other pigmented purpuric dermatoses. It more frequently affects middle-aged women of Asian origin, and predominantly on the lower extremities. The authors present the case of a female patient with granulomatous pigmented purpuric dermatosis on the lower extremities with blaschkoid distribution.

Poroma no pigmentado inusualmente grande: reporte de un caso y revisión de la literatura / Unusually large unpigmented poroma: a case report and literature review

El poroma es un tumor benigno infrecuente derivado de la porción intraepitelial del epitelio ductal de la glándula sudorípara. La presentación clínica más habitual es un pápula o nódulo de color rosado a rojo, de pequeño diámetro, localizado con mayor frecuencia en palmas y plantas, generalmente asintomático. Debido a su variabilidad morfológica, los poromas son usualmente difíciles de reconocer, siendo la dermatoscopía una herramienta útil en su diagnóstico. Presentamos el caso de un paciente masculino con un gran tumor en su pierna derecha de 40 mm de diámetro, con características dermatoscópicas compatibles con poroma no pigmentado. El diagnóstico confirmatorio se realizó con una biopsia incisional seguido de la extirpación quirúrgica completa del tumor

Poroma is a rare benign tumour derived from the intraepithelial portion of the sweat glands' ductal epithelium. The most common clinical presentation is a pink to red papule or nodule, small in diameter, predominantly in the palms and soles and generally asymptomatic. Due to its morphologic variability, poromas are usually difficult to recognize, thus dermatoscopy becomes a useful aid in its diagnosis. We present the case of a male patient with a large tumour on his right leg, 40 mm in diameter, with dermatoscopic features consistent with nonpigmented poroma. Confirmatory diagnosis was made by incisional biopsy followed by the total surgical excision of the tumor.

Infección por Serratia marcescens: una inusual causa de lesiones cutáneas / Serratia marcescens infection: an unusual cause of skin lesions

Serratia marcescens corresponde a un bacilo gram negativo, miembro de la familia Enterobacteriaceae. Este microorganismo tiene una alta capacidad de supervivencia en condiciones hostiles y ha sido implicado en infecciones del tracto respiratorio, vía urinaria, meningitis, endocarditis y sistema musculoesquelético. No obstante, es considerado una causa rara de infecciones cutáneas. Esta última tiene distintas presentaciones clínicas, la más frecuente es fascitis necrotizante seguida de celulitis. Los nódulos, las pápulas después de inyecciones de rellenos, las erupciones papulares diseminadas, las placas eritematosas, las pústulas y las úlceras son parte del amplio espectro de formas clínicas descritas en la literatura. Presentamos el caso de una paciente de 50 años, con historia de compromiso del estado general, lesiones cutáneas polimorfas y fiebre. Se confirmó una infección cutánea por Serratia marcescens mediante cultivos. Se destaca el polimorfismo y la coexistencia de distintas manifestaciones en una misma paciente, incluyendo celulitis, nódulo, ulceras y necrosis cutánea y la importancia del estudio microbiológico para el adecuado tratamiento antibiótico.

Serratia marcescens corresponds to gram negative bacillus, a member of the Enterobacteriaceae family. This microorganism has a high survival capacity in hostile conditions and has been implicated in respiratory tract, urinary tract, meningitis, endocarditis and musculoskeletal system infections. However, it is considered a infrequent cause of cutaneous infections. Has different clinical presentations, the most frequent is necrotizing fasciitis followed by cellulite. Nodules, papules after filler injections, disseminated papular eruption, erythematous plaques, pustules and ulcers are part of the broad spectrum of clinical forms described in the literature. We present the case of a 50 year old patient with a history of compromised general condition, polymorphic cutaneous lesions and fever. Serratia marcescens cutaneous infection was confirmed by cultures. The polymorphism and the coexistence of different manifestations in the same patient, including cellulitis, nodule, ulcers and skin necrosis, and the importance of the microbiological study for the adequate antibiotic treatment are highlighted.

Síndrome de Proteus: reporte de un caso y revisión de la literatura / Proteus syndrome: a case report and literature review

El síndrome de Proteus corresponde a una entidad poco frecuente caracterizada por un sobrecrecimiento progresivo de piel, tejido óseo y adiposo, debido a una mutación somática activante del gen AKT1. Existen distintas manifestaciones cutáneas entre las que se incluyen nevo cerebriforme de tejido conectivo plantar, nevo epidérmico, malformaciones vasculares y trastornos del tejido adiposo que pueden alertar al dermatólogo para poder diagnosticar esta condición, permitiendo un manejo precoz que impida el desarrollo de complicaciones y la muerte temprana. Presentamos el caso de una paciente de 9 años cuya historia clínica y examen físico reflejan los hallazgos clásicos del síndrome de Proteus, recalcando la importancia de un manejo multidisciplinario oportuno.

Proteus syndrome is a rare condition characterized by a progressive overgrowth of skin, bone tissue and adipose tissue, due to an activating somatic mutation of the AKT1 gene. Different cutaneous manifestations that include cerebriform connective tissue nevi, epidermal nevus, vascular malformations and adipose tissue disorders can alert the dermatologist to diagnose this condition, allowing an early management that prevents the complications and early death. We present the case of a 9-year-old patient whose clinical history and physical examination reflect the classic findings of Proteus syndrome, highlighting the importance of a multidisciplinary management.

Utilidad de tinción de tricrómico de Masson en la cuantificación de densidad media vascular en mucosa oral normal, displasia epitelial y carcinoma oral de células escamosas / Utility of Masson's trichrome stain in the quantification of mean vascular density in normal oral mucosa, epithelial dysplasia and oral squamous cell carcinoma

RESUMEN: El objetivo de este estudio fue valuar la utilidad del uso de la tinción de Tricrómico de Masson (TM) en la cuantificación de la densidad media vascular (DMV) en Mucosa Oral Normal (MON), Displasia Epitelial Oral (DEO) y Carcinoma Oral de Células Escamosas (COCE). Estudio descriptivo de serie de casos. Se analizaron 17 muestras de MON, 15 muestras de DEO y 16 de COCE, teñidas con TM. Para determinar su utilidad, se compararon con las mismas muestras analizadas con técnica de inmunohistoquímica contra CD31. La cuantificación de la DMV se realizó en las 3 áreas de mayor vascularización de cada muestra. Se determinó la DMV según diagnóstico mediante la tinción TM e inmunohistoquímica contra CD31, y se calculó la correlación entre ambos. La DMV cuantificada con TM y contra CD31 difiere según el diagnóstico, observándose un aumento de la DMV al malignizarse el diagnóstico. No se encontraron diferencias al comparar la DMV cuantificada con TM y contra CD31. La correlación de la DMV analizado por TM y contra CD31 es significativa y moderada. La cuantificación de vasos sanguíneos es posible mediante la tinción de TM en muestras de MON, DEO y COCE, con una correlación moderada con la inmunohistoquímica contra CD31.

SUMMARY. The objective of this study was to evaluate the utility of Masson's Trichrome (TM) staining in the quantification of the mean vascular density (DMV) in samples of normal oral mucosa (MON), oral epithelial dysplasia (ODE) and oral squamous cell carcinoma (COCE). The design - a descriptive study of case series. We analyzed 17 samples of MON, 15 samples of DEO and 16 samples of COCE, stained with TM. To determine usefulness, we compared and analyzed the same samples, either stained with TM or with immunohistochemical technique against CD31. Quantification of the DMV was performed in the 3 areas of greatest vascularization in each sample. DMV was determined according to diagnosis by TM staining and immunohistochemistry against CD31, and the correlation between the two was then calculated. DMV quantified with TM and against CD31 differs according to the diagnosis, with an increase in DMV upon malignant diagnosis. No differences were found when comparing DMV quantified with TM and against CD31. The correlation of the DMV analyzed by TM and against CD31 is significant and moderate. Quantification of blood vessels is possible by TM staining in samples of MON, DEO and COCE. TM staining is moderately correlated with immunohistochemistry against CD31.

Validación de la escala de bienestar mental de Warwick - Edingurgo, en Chile / Validação da escala de bem-estar mental de Warwick Edimburgo, no Chile / Validation of Warwick-Edinburgh mental wellbeing scale in Chile

Introducción: El bienestar mental es un constructo relevante,pero ha sido poco estudiado y existen muy pocos cuestionariospara su valoración. Objetivo: Adaptar y validar la versiónespañola de la Escala de Bienestar Mental de WarwickEdimburgo.Material y Método: Se realizó un procedimientode validación semántica, seguido de una aplicación a 220personas adultas para estudiar sus propiedades psicométricas.Resultados: Se introdujeron cambios semánticos mínimos.Se utilizó un análisis factorial de componentes principales,con buenos resultados (KMO= 0,897) y una muy buenaconsistencia interna para un factor(∝ = 0,875). Estecuestionario mostró una buena correlación con el GHQ-12 y el cuestionario de calidad de vida WHOQOL-BREF.La correlación test - retest fue positiva y estadísticamentesignificativa (r = 0,556, p < 0,001). Conclusión: Esta escalamostró buenos indicadores psicométricos, siendo unaherramienta útil y fácil de aplicar para la evaluación delbienestar mental en adultos.

Introduction: Mental well-being is a relevant construct,but it has not been thoroughly studied and there are very few assessment questionnaires. Objective: Adapt and validate the Spanish version of theWarwick-Edinburgh Mental Well-Being Scale. Material and Method: A process of semanticvalidation was performed, followed by the application to 220 adults in order to study theirpsychometric properties. Results: Minimal semantic changes were introduced. Factorialanalysis of the main components was used, with good results (KMO= 0.897) and verygood internal consistency for a factor (∝ = 0.875). This questionnaire showed very goodcorrelation with questionnaire GHQ-12 and the one on life quality WHOQOL-BREF.The correlation test-retest was positive and statistically significant (r = 0.556, p < 0.001).Conclusion: This scale showed good psychometric indicators, being a simple and easy toapply tool to assess mental well-being in adults.

Modelo explicativo del efecto de la talla y grasa corporal en el peso de escolares entre 9 y 17 años de edad / Explanatory model of the effect of size and body fat in the weight of school children between 9 to 17 years

La obesidad ha aumentado en niños y jóvenes. La importancia de medir la composición corporal es reconocida. Sin embargo, la talla y la proporción de grasa podrían explicar la variabilidad del peso y dependerían de la edad y sexo de la persona. Objetivo Analizar la relación de la talla y grasa corporal con el peso de los estudiantes. Diseño Estudio observacional, transversal y analítico. Lugar y sujetos Se empleó la base de datos del estudio "Quito municipal schools cohort study: Baseline results", desarrollado en 6964 escolares de 9 a 17 años. Mediciones principales Se consideró las variables edad, sexo, peso, talla y grasa corporal. La condición antropométrica fue definida según criterios de la Organi- zación Mundial de Salud. Para evaluar la correlación entre el peso en función de la talla y proporción de grasa corporal se aplicó un modelo de regresión lineal múltiple. Resultados La correlación entre el peso, talla y grasa corporal fue significativa, tanto en mujeres (r=0.93) como en varones (r=0.92). El coeficiente de determinación disminuyó con la edad y la aportación parcial de la grasa al peso fue mayor hasta los 11 años en mujeres y 14 años en varones. En los varones con exceso de peso la aportación de la grasa para explicar el peso fue menor que la talla y en las mujeres la aporta- ción de la grasa corporal y la talla fue igual en todas las edades. Conclusión La proporción de grasa debería tenerse en cuenta siempre que se de- termine el exceso de peso y se determine y analice el sobrepeso y la obesidad, porque es el único de los dos términos que se puede modificar

Obesity has increased in children and youth. The importance of measuring body composition is known. However, the size and the proportion of fat could explain weight variability and depend on the age and sex of the person. Objective To analyze the relationship between size and amount of body fat and the weight of the students. Design Cross ­ sectional study Subjects and setting The database from the study "Quito municipal schools cohort study: Baseline results" was used. This study was developed in 6964 students aged 9 to 17 years. Main measurements Age, sex, weight, height and body fat were considered in the analysis. Anthropometric status was defined according to criteria of the World Health Organization. To evaluate the correlation between the weight depending on the size and proportion of body fat a multiple linear regression was applied. Results The correlation between weight, height and body fat was significant both in women (r=0.93) and in men (r=0.92). The coefficient of determi- nation decreased with age and the partial contribution of fat to weight was higher until the age of 11 in women and 14 years in men. In males with overweight the contribution of fat to explain the weight was lower than the height and in women the contribution of body fat and the height was the same in all ages. Conclusion The proportion of fat should be taken into account when excess weight is determined and when overweight and obesity are analyzed because it is the only one of the two terms that can be modified. Abstract Explanatory model of the effect of size and body fat in the weight of school children between 9 to 17 years 

Nivel de riesgo materno según AtEPI Neonatal y su asociación a óbitos fetales, morbilidad y mortalidad neonatal en tres unidades hospitalarias de la ciudad de Quito / Level of risk as maternal Atepi Neonatal deaths and its association with fetal, neonatal morbidity and mortality in three hospitals in the city of Quito

Objetivo: Identificar el nivel de nesgo materno y relacionado a la prevalencia de óbitos fetales, mortalidad y morbilidad neonatal. Métodos: Estudio transversal, se revisaron 298 historias clínicas de neonatos fallecidos, neonatos ingresados a Unidad de Cuidados Intensivos Neonatales y madres con diagnóstico de óbito fetal, en tres casas de salud de la ciudad de Quito que cuentan con servicios de Neonatología y Ginecología, durante el primer semestre del 2007. Se empleó como instrumento de clasificación del riesgo materno el algoritmo que consta en el manual de Atención Integrada a las Enfermedades Prevalentes de la Infancia Neonatal. Resultados: Se identificaron a 219 madres con embarazo de alto riesgo, 69 con mediano y 10 con mínimo riesgo. Se encontró asociación entre madres con embarazo de alto riesgo y defunciones neonatales (OR 8.45 IC95 por ciento 3.38-21.14) y neonatos ingresados a Unidad de Cuidados Intensivos Neonatales (OR 4.43 IC95 EC1P1.046 2.06-9.53). Conclusión: La identificación del nivel de riesgo materno a través del algoritmo de Atención Integrada a las Enfermedades Prevalentes de la Infancia Neonatal permitió asociar el alto riesgo con morbilidad y mortalidad neonatal.